Laboratories

Topics of Physiology labs, winter semester 2024

Winter semester lab topics

L1

  1. Counting of red blood cells
  2. Estimation of hemoglobin concentration acc. to Drabkin
  3. Various derivatives of hemoglobin
  4. Hematocrit
  5. Characteristics of red blood cell

L2

  1. Counting of leukocytes
  2. Differential leukocyte count
  3. Blood analysis with Hemalog
  4. Counting of thrombocytes acc. to Fonio
  5. Counting of reticulocytes
  6. Influence of various solutions on erythrocytes

 L3

  1. Blood typing – AB0 groups
  2. Blood typing – Rh
  3. Pre-transfusion serologic test (LISS and LEN)
  4. Erythrocyte sedimentation rate “micro” method

 L4

  1. Prothrombin time measurement acc. to Quick
  2. Clotting time measurement acc. to Lee-White
  3. Measurement of clot retraction
  4. Bleeding time measurement acc. to Duke
  5. Estimation of erythrocytes osmotic fragility acc. to Sanford

 L5

  1. Registration of action potentials of the heart (ECG).
  2. Influence of Valsalva maneuver and exercise on the ECG.
  3. Determination of electrical axis of the heart.
  4. Measurement of blood pressure and pulse. Pulse characteristics.
  5. Cardiac cases.

L6

  1. Animal experiments – virtual physiology
  2. Blood physiology with use of computer programs

 

Spring semester lab topics

L7

  1. Reaction of blood vessels to ischemia and hyperemia.
  2. Carotid sinus reflex.
  3. Vasomotor reactions in skin caused by mechanical stimulus (dermographism).
  4. Influence of gravity and temperature on arterial pressure and heart rate.
  5. Examination of apex beat.
  6. Auscultation of heart sounds.
  7. Cases regarding cardiovascular system.

L8    

  1. Measurement of vital capacity.
  2. Measurement of forced expiratory volume (FEV1).
  3. Peak expiratory flow (PEF).
  4. Voluntary apnea time and calculation of the minimum expiratory volume.
  5. Cases regarding respiratory system.

L9

  1. Examination of touch and pain sensation.
  2. Examination of vibration (deep) sensation
  3. Examination of touch discrimination.
  4. Examination of coordination.
  5. Examination of postural reflexes testing the labyrinth excitability.
  6. Examination of unconditioned reflexes.
  7. Examination of postural efficiency by means of Fukuda postural test.

L10

  1. Examination of visual acuity.
  2. Visual field.
  3. Presentation of blind spot.
  4. Examination of afterimages.
  5. Ambiguous figures.
  6. Examination of color vision.
  7. Examination of hearing.
  8. Tuning fork tests.
  9. Measurements of reflex time for light and acoustic stimuli.

 L11

  1. Measurement of physical efficiency in humans: Harvard test, Martinet test.
  2. Estimation of physical work capacity with W170 index.
  3. Assessment of work load.
  4. Estimation of maximal oxygen uptake (VO2max).
  5. Adaptive changes in cardiovascular system under physical effort of various types (endurance and speed effort).
  6. Muscle fatigue during isotonic and isometric contractions.
  7. Estimation of appropriate body mass and Body Mass Index.
  8. Water balance.

L12

        Class in EaMSC – patient simulator

L13

        "Practical exam" - assessment of labs, mandatory pass mark before the final exam